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standard by International Organization for Standardization, 04/01/2013
ISO 23893-3:2013 specifies a method for measuring vitellogenin (Vtg) concentrations in a fish plasma sample employing an enzyme-linked immunosorbent assay (ELISA) method.
It applies to fish that are sampled in the environment (fresh, estuarine or salt water) and to fish exposed to substances or effluents in a laboratory. The method is quantitative when using Vtg antibodies and a Vtg standard well characterized with the species of choice.